Proposed Lab Experiment V. Effect of different compounds on bee mortality and parasite levels

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Introduction

Honeybee guts 1000x Small divisions are 1 um.
Insect wing scale


Feed groups of bees stressors, nutrients or treatments and measure mortality rates and parasite loads.


  1. Crithidia mellificae
  2. Nosema ceranae

References


Ingemar Fries, Marie-Pierre Chauzat, Yan-Ping Chen, Vincent Doublet, Elke Genersch, Sebastian Gisder, Mariano Higes, Dino P McMahon, Raquel Mart ín-Hernández, Myrsini Natsopoulou, Robert J Paxton, Gina Tanner, Thomas C Webster & Geoffrey R Williams (2013) Standard methods for Nosema research, Journal of Apicultural Research, 52:1, 1-28, DOI: 10.3896/IBRA.1.52.1.14


Chen Y1, Evans JD, Smith IB, Pettis JS. https://www.ncbi.nlm.nih.gov/pubmed/17880997 https://www.sciencedirect.com/science/article/abs/pii/S002220110700153X?via%3Dihub


Dong-Jun Kim, Hwi-Geon Yun, In-Hui Kim, Won-Seok Gwak, and Soo-Dong Woo Efficient Method for the Rapid Purification of Nosema ceranae Spores https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673517/


Eric C. Mussen, Extension Apiculturist, UC Davis (March 11, 2011) Diagnosing and Treating Nosema Disease http://entomology.ucdavis.edu/files/147621.pdf


https://www.sciencedirect.com/science/article/pii/B9780123849847000129


Standard methods for fungal brood disease research

rust fungi spores

A Brazilian Social Bee Must Cultivate Fungus to Survive

Texas Apiary Inspection Service

cyberbee.net

Resources

trypsin https://www.labsupplyoutlaws.com/chemicals-and-reagents/trypsin-powder-125-units-gram.htm

collagenase http://www.worthington-biochem.com/cls/pl.html

Toluidine Blue O https://www.carolina.com/catalog/detail.jsp?prodId=896638